Abstract
Cobicistat is a cytochrome P450 3A (CYP3A) inhibitor. It boosts blood levels of the HIV protease inhibitors Atazanavir and Darunavir by suppressing CYP3A, an enzyme that metabolizes these drugs in the body. Cobicistat acts only as a pharmacokinetic enhancer and has no antiviral activity. In the present basic work a rapid, simple and economic UV spectrophotometric method has been developed for Cobicistat in its bulk form using 0.1N HCl. The λmax was determined to be 246.2nm. The method was validated and proved to be linear in the range of 10-150ug/ml, exhibited good correlation coefficient (R2=0.9998). The validated method was found to be precise and robust. Thus this simple basic method can be used for the determination of cobicistat.
