Abstract
An isocratic Simultaneous estimation by RP-HPLC Method were developed and validated for the quantification of OLANZAPINE and FLUOXETINE HYDROCHLORIDE in tablet dosage form. Quantification was achieved by using a reversed-phase C18 column (Inertsil C18 Column , 5μ, 250 mm × 4.6 mm) at ambient temperature with mobile phase consisting of 0.1%v/v Ortho Phosphoric acid in water (pH 3.5 With Triethylamine):Acetonitrile: Methanol (60:30:10). The flow rate was 1.0 ml/min. Measurements were made at a wavelength of 225nm. The average retention time for OLANZAPINE and FLUOXETINE HYDROCHLORIDE were found to be 2.19 min and 3.71. The proposed method was validated for selectivity, precision, linearity and accuracy. The assay methods were found to be linear from 12-28μg/ml for OLANZAPINE and 48-112μg/ml for FLUOXETINE HYDROCHLORIDE. All validation parameters were within the acceptable range. The developed method was successfully applied to estimate the amount of OLANZAPINE and FLUOXETINE HYDROCHLORIDE in tablet dosage form.
