Abstract
A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for the simultaneous determination Metolazone and Spironolactone in pharmaceutical dosage form. The column used was Altima C18 (150mm x 4.6 mm, 5) in isocratic mode, with mobile phase containing phosphate buffer and acetonitrile (42:58 v/v). The buffer is prepared by adding 1.36gm of potassium dihyrogen ortho phosphate in a 1000ml of volumetric flask add about 900ml of milli-Q water added and degas to sonicate and finally make up the volume with water then pH adjusted to 4.8 with dil. orthophosphoric acid solution. The flow rate was 1.2ml/ min and effluents were monitored at 230 nm. The retention times of Metolazone and Spironolactone were found to be 2.121 min and 4.873 min, respectively. The linearity for Metolazone and Spironolactone were in the range of 12.5-75 μg/ml and 125-750 μg/ml respectively. The recoveries of Metolazone and Spironolactone were found to be 98.45% to 99.68% w/v and 98.85% to 101.14%w/v, respectively. The proposed method was validated and successfully applied to the estimation of Metolazone and Spironolactone in combined tablet dosage forms.
