Abstract
A new simple accurate and economical reverse phase high performance liquid chromatographic method was developed for the determination of Thiocolchicoside and Flupirtine Maleate in bulk and tablet dosage form. The separation was eluted on a C18 column (250 mm x 4.6 mm; 5μ) using a mobile phase mixture of mixed phosphate buffer 6.5 and acetonitrile in a ratio of 50:50 v/v at a flow rate of 1.0ml/min. The detection was made at 255 nm. The retention times were 1.96min for Malic acid, 2.52min for Thiocolchicoside and 4.97min for Flupirtine. Calibration curve was linear over the concentration range of 4-24 μg/ml for Thiocolchicoside and 50 to 300 μg/ml for Flupirtine. The propose method was validated as per the ICH guidelines parameters like Linearity, specificity, precision, accuracy, robustness and ruggedness. The method was accurate, precise, specific and rapid found to be suitable for the quantitative analysis of the drug and dosage form.
