Abstract
A simple reverse phase – HPLC method was developed and validated for the estimation of diazepam in bulk and tablet dosage forms. Isocratic elution at a flow rate of 1.0 mL/min was employed on BDS Hypersil C18 (250 X 4.6) at ambient temperature. A mixture of 0.05M formic acid, methanol and acetonitrile in a ratio of 45:20:35 (v/v) was used as the mobile phase. The UV detection wavelength was 239nm and the sample size was 20μL. The retention time for diazepam in this method was 5.272min. Linearity was obtained in the range of 40-100 μg/mL for diazepam. The mean recovery of diazepam was found to be 99.93%. The method was validated as per the ICH guidelines. The validated method was found to be precise and accurate for the estimation of diazepam in tablet dosage forms.
