Abstract

Traditional system of medicine has got an overwhelming response during recent years. Across the world these medicaments have been used by people to support chronic ailments like arthritis, diabetes mellitus, respiratory disease and some chronic skin infections. Kaisoraguggulu is one of the traditional recipe mentioned in authoritative ayurvedic texts have found to support chronic skin infections, to control inflammation in gout, cleanses blood etc, due to global demand for herbal preparations a robust manufacturing methods and standardization of marker compounds specially for polyherbal formulations which can assess batch to batch consistency in quality and quantity is the need of the hour. This can be accomplished by modern scientific tools which can ensure the quality, efficacy and safety of the product. To address this, novel and simple analytical method for Piperine, Guggulusterone and Embelin using RP-HPLC method was developed.The elution was carried out using Chromolith® RP-18 (100 x 4.6 mm) 3μm pore size column, mobile phase consisted of 80% methanol and 0.05m phosphate buffer, with gradient elution, compounds were eluted at 6.0, 8.38,10.87 and 16 minutes for piperine, guggulusterone E & Z and embelin respectively. The detection of analytes was at 254nm for Piperine, 291nm for Guggulusterone and 340nm for Embelin. The established and validated method can be applied for quantification of above said compounds during bulk manufacturing.