Abstract
A simple, specific, accurate RP-HPLC method was developed for simultaneous determination of Amitriptyline, Carbamazepine and Flunarizine in human plasma using C8 (250 × 4.6 mm, 5μ) Column and a mobile phase composing of methanol, acetonitrile and phosphate buffer (pH 3) in proportion of 50:40:10 (v/v). Mitrazapin was used as Internal Standard. The flow rate was 0.8 ml/min and the effluents were monitored at 210 nm. The retention time of Amitriptyline, Carbamazepine and Flunarizine were 3.6 ±0.1, 4.1±0.2 and 4.6±0.2 min respectively. Quantitative and recovery studies of the dosage form were also carried out and the % RSD was found to be less than 1. The developed method is simple, rapid and accurate and hence can be used for routine quality control analysis.
