Abstract
Formulation of chemotherapeutics of platinum compounds has become challenging mainly due to their physicochemical properties. Utilization of nano carrier approach for delivery of oxaliplatin enables in decreasing the side effects to healthy cells by targeting the drug to tumors. Along with targeting, the idea behind this study is also to increase the stability and therapeutic index of oxaliplatin compared to the conventional formulation by formulating as solid lipid nanoparticles (SLN). In this study, micro emulsion method was employed for preparing SLN (with different ratio of lipid), which can be used in industrial production conveniently and characterized by dynamic light scattering and transmission electron microscopy for size and morphology. Differential scanning calorimetry and X-ray diffraction studies revealed that the drug was dissolved in the blend of glycerol monostearate. Particle size and zeta potential was found to be 127.8±4.4 nm and -29.8±2.30 mV respectively for the optimized batch with drug: lipid ratio of 1:10. Encapsulation efficiency was performed using an ultra filter and determined by high performance liquid chromatographic method which was found to be 64.21% for the optimized batch. The in vitro release experiments of Oxaliplatin SLN exhibited release pattern with burst release at initial 2.5 hours and then after the release was sustained for prolonged effect contrary to the 90% drug release in oxaliplatin pure solution. The results indicated that the Solid lipid nanoparticles obtained in this study was a potential carrier for the delivery of Oxaliplatin to the colorectal tumors effectively.
