Abstract
The present study describes two simple, sensitive, accurate, rapid, and economical Visible Spectrophotometric methods were developed for the assay of Flupirtine maleate in pure and pharmaceutical dosage forms. In this, the Method ‘A’ was developed based on oxidative coupling of Flupirtine maleate with MBTH reagent in the presence of FeCl3 (0.2%) giving a pink-coloured chromogen, which showing maximum absorbance at 535 nm against reagent blank, while in the Method ‘B’, based on the reaction of Flupirtine maleate with 2,2-Bipyridyl in the presence of FeCl3(0.1%) giving a red-coloured chromogen , which showing maximum absorbance at 522 nm against reagent blank. The both reaction conditions were optimized and Beer’s law was obeyed in the range of 2 to 10 μg/ml for Method A, 3 to 15μg/ml for Method B with the correlation coefficients of 0.9999. The percentage recovery was also found to be in the range of 99.82-100.25% for method A and 99.53-100.53% for method B. The LOD and LOQ values were found to be 0.4 µg/ml & 1.2 µg/ml for method A and 0.06 µg/ml & 0.18 µg/ml for method B, respectively. The both methods were validated in accordance with the current ICH guidelines. The % accuracy was ranged between 99.86 to 100.33 for Method A and 99.53 to 100.53 for Method B. No interference was observed from common pharmaceutical excipients. The % RSD for inter day and intraday precision of the both methods were found to be less than 2. Hence it could be concluded that the developed Visible Spectrophotometric methods would be suitable for the analysis of Flupirtine maleate in bulk and pharmaceutical formulations containing various excipients.
