A new simple, precise and accurate ultra performance liquid chromatographic method was developed and validated for the estimation of udenafil in tablet dosage form. In this method, Chromatographic separation was achieved by injecting a volume of 0.5μl of standard udenafil drug into HSS C18, (100 mm x 2.1x 1.8 μm) column and mobile phase of composition 500 mL of solution A (Dissolved 1.36 g of KHP2PO4 in to 1 lit hplc grade water, pH adjusted to 2.3 with dil OPA solution, filtered using 0.22μm filter) and 500 mL of solution B (acetonitrile and methanol in 9:1 ratio) was allowed to flow through the column at a flow rate of 0.3 ml per minute. Detection of the component was carried out at a wavelength of 235nm. The retention time for udenafil was found to be 0.97 min. The developed method was validated for parameters like systems suitability, Linearity, accuracy, specificity, LOD & LOQ and robustness as per ICH guidelines. All the results obtained were within the limits; hence it can be employed for routine quality control of udenafil tablets in drug testing laboratories and pharmaceutical industries.
