Two new, simple and sensitive spectrofluorimetric methods have been developed for the estimation of ethambutol hydrochloride in bulk and oral dosage form. Method A involves the determination of ethambutol hydrochloride by direct spectrofluorimetric method the relative fluorescence intensity was measured an emission wave length ( λem ) of 521nm with an excitation wave length ( λex ) at 385nm; the fluorescence concentration range was found to be 0.2-1.0μg/mL; Method B involves the determination of ethambutol hydrochloride by oxidative spectrofluorimetric method using potassium permanganatethe relative fluorescence intensity was measured an emission wave length ( λem ) of 469nm with an excitation wave length ( λex ) at 405nm; the fluorescence concentration range was found to be 0.2-1.0μg/mL. The correlation coefficients for the methods were found to be 0.998 & 0.999. The methods showed good reproducibility and recovery. So the proposed methods were found to be simple, precise and accurate. The results of analysis have been validated statistically by ANOVA method and by recovery studies.