Glycosmis pentaphylla (Retz.,)DC (Rutaceae) is an important traditional medicinal plant being used for treatment of cough, rheumatism, anemia, antioxidant and jaundice. Systematic pharmacognostical evaluation of the root of the plant has been carried out with respect to macroscopy, microscopy, physicochemical parameters and estimation of different standards. TLC profiles were developed for petroleum ether, chloroform, ethyl acetate and ethanol extracts. The preliminary phytochemical investigations indicated presences of alkaloids, terpenoids, flavanoids, tannins, sugar, glycoside and phenolic compounds. The results obtained are useful for standardization of the root established the macro, micro, powder microscopy and physiochemical parameters to characterize the genuine plant drug. This parameter can be utilized for quick identification of the drug and are particularly useful in the case of powdered forms and also contribute towards establishing pharmacopoeial standards for the specified plant. Glycosmis pentaphylla possesses medicinally important secondary metabolites; these can be employed in the treatment of various diseases.
