Abstract
It is to determine the anticlastogenic activity of Hydroalcoholic Extract of Mangifera indica Linn. leaves, stem bark and fruit skin (HEMI) on chromosomal damage induced by Cyclophosphamide (CP) by in vivo mammalian Chromosomal aberration test in mice. In the present investigation, eight groups of swiss albino mice six in each group were used. Except the first group all the remaining groups were treated with CP (40 mg/kg). Group I serve as control. Group II was treated with CP. Mice of III, IV and V groups were treated with HEMI at 50,100 and 150 mg/kg doses respectively along with CP. Mice of VI, VII and VIII groups were treated with HEMI at 50,100 and 150 mg/kg doses respectively without CP. Metaphase of bone marrow cells of all animals were analyzed for structural and numerical chromosomal aberrations. Break, gap, fragment, association, deletion, polyploidy, pulverization, ring, other multiple aberrations, total aberrations, total aberrated cells and mitotic index were observed. Groups III and IV showed significant (P<0.001) decrease in chromosomal aberrations when compared to CP group. Groups III, IV and V showed 66.77%, 60.04% and 50.26 % protection against CP induced chromosomal aberrations respectively. Total chromosomal aberrations were found to be 431, 367 and 524 in III, IV and V groups respectively. From the present study it can be observed that HEMI possess significant anticlastogenic activity against CP induced chromosomal aberrations.
