Abstract

To compare anti diabetic effect of Methanolic extract of Insulin plant (Costus pictus) leaves (MECP) and its silver nanoparticle (MECPAgNPs) by various in vitro screening methods. Preliminary phytochemical screening, Vitamin C, total phenolic and flavonoid content were determined by standard procedure. TLC and HPTLC (quantitative) analysis of MECP was done by using Quercetin as a standard. Screening of in vitro anti diabetic activity was done by Inhibition of α-amylase & α-glucosidase enzyme assay, Glucose uptake in yeast cells and Non-enzymatic glycosylation of Hemoglobin assay. Preliminary phytochemical screening showed the presence of carbohydrates, triterpenoids, proteins, alkaloids, tannins, saponins, flavonoids, sterols and volatile oil. Phenolic, Flavonoid content and Vitamin C of MECPAgNPs was higher than MECP. TLC analysis of MECP confirmed the presence of Quercetin and its amount was found to be 115.41mg/g by HPTLC. Non-enzymatic glycosylation of Hemoglobin assay, MECPAgNPs exhibited higher inhibition of glycosylation as compared with the MECP at a concentration of 200-1000µg/ml. In α-amylase and α-glucosidase enzyme inhibition assay, the results showed MECPAgNPs had efficiently inhibit both enzymes, in vitro in dose dependent manner than MECP at a concentration of 200-1000µg/ml. It can be concluded that the leaves of C.pictus D. Don can be a good source for synthesis of silver nanoparticle which shows potent anti oxidant and anti diabetic activity than the MECP. The important outcome of the study will be the development of value added product from medicinal plant Costus pictus D. Don for biomedical and nanotechnology based industries. Furthermore, in vivo studies are required for providing scientific information on the plant.