To develop and validate a simple accurate method for simultaneous estimation of Meclizine Hydrochloride (MEC) and Nicotinic Acid (NA) which can be applied for their dosage forms & biological samples. The method was carried out on Agilent C18 (25 cm x 4.6 mm i.d., 5 μm) column with a mobile phase consisting of Methanol : Water (0.2% TEA, adjusted to pH 3.0 using orthophosphoric acid) in the ratio of 80:20 v/v. The retention time of Meclizine Hydrochloride and Nicotinic Acid is 3.38 min and 5.50 min respectively with the flow rate of 1ml/ min with VWD detection at 231 nm. The linear regression analysis data for the linearity plot showed good linear relationship with correlation coefficient value for Meclizine Hydrochloride and Nicotinic Acid were r2=0.9991 and r2=0.9996 in the concentration range of 5-35 μg/ml, 10-70 μg/ ml respectively. The relative standard deviation for intra-day precision has been found to be lower than 2.0 %. The method is validated according to the ICH guidelines. The developed method is validated in terms of specificity, selectivity, accuracy, precision, linearity, limit of detection, limit of quantification and solution stability. The developed method can be successfully applied for the determination of these drugs in combined dosage forms and in Plasma.
