Medicinal plants are extensively used all over the globe has led to a rise in major concerns over the quality, wellbeing and effectiveness of the natural products they have.  Ailanthus excels Roxb. is a tree,  that is native to the central and southern regions of India. This plant belongs to Simaroubaceae family. This plant is commonly called as “Tree of Heaven”. In the present study leaf and stem parts were extracted with petroleum ether, benzene, chloroform, acetone, ethanol and water according to the polarity of these solvents for the extractive value. Maximum yield in leaf part was obtained in water extract (7.64%).  In stem extract, maximum yield was also observed in water extract (2.91%). These parts were also quantitatively estimated and found that leaf part has more concentration of primary metabolites than stem part except protein (36.83±2.70 mg/g dw) and phenol (9.06±0.23  mg/g dw) amount. Aerial part of the plant were subjected to fluorescence analysis treating with different chemicals which produce different colors. These analyses are essential for the formation of drugs.

1. Najmi A, et al. Modern approaches in the discovery and development of plant-based natural products and their analogues as potential therapeutic agents. Molecules. 2022;27(2):349.
2. Panchawat S, Ameta C. Medicinal importance of plant metabolites. In: Chemistry of Biologically Potent Natural Products and Synthetic Compounds. Hoboken (NJ): Wiley; 2021. p. 1–19.
3. Singh AK, Dhanapal S, Yadav BS. The dynamic responses of plant physiology and metabolism during environmental stress progression. Mol Biol Rep. 2020;47:1459–70.
4. Said M, et al. In vitro antioxidant and antiproliferative activities of flavonoids from Ailanthus excelsa (Roxb.) leaves. Z Naturforsch C Biosci. 2010;65(3-4):180–6.
5. Patil DA. Flora of Dhule and Nandurbar Districts (Maharashtra). Dehradun: Bishen Singh Mahendra Pal Singh; 2003.
6. Nadkarni KM. Indian Materia Medica. Vol. 1. Mumbai: Popular Prakashan; 1996.
7. Tayade SK, Pathak SS, Patil VK. Ethnobotanical study of some useful plants of Nandurbar District (Maharashtra). Int J Sci Res. 2016;5(1):160–2.
8. Tayade SK, Patil DA. Hitherto untapped plantflore from Nandurbar district (Maharashtra). Nat Prod Radiance. 2005;4(1):46–50.
9. Ahirrao YA, Patil DA. Ethnomedicinal investigation in Nandurbar district of Maharashtra. Anc Sci Life. 2007;27(2):50–6.
10. Kirtikar KR, Basu BD. Indian Medicinal Plants. Vol. 1. Dehradun: International Book Distributors; 1995. p. 371–2.
11. Chevallier A. The Encyclopedia of Medicinal Plants. London: Dorling Kindersley; 1996.
12. Pramila G, Jirekar D. Phytochemical, spectral analysis and metal content of Ailanthus excelsa leaves. World J Pharm Life Sci. 2024;10(4):200–5.
13. Cullen E. Novel anti-inflammatory agents. J Pharm Sci. 1984;73(5):579–89.
14. Shrimali M, et al. Antibacterial activity of Ailanthus excelsa (Roxb). Phytother Res. 2001;15(2):165–6.
15. Joshi B, et al. Antifungal activity of the stem bark of Ailanthus excelsa. Fitoterapia. 2003;74(7-8):689–91.
16. Ravichandran V, et al. Antifertility activity of hydroalcoholic extract of Ailanthus excelsa (Roxb). J Ethnopharmacol. 2007;112(1):189–91.
17. Dell’Agli M, et al. Anti-plasmodial activity of Ailanthus excelsa. Fitoterapia. 2008;79(2):112–6.
18. Khan S, Shamsuddin K. Isolation and structure of 13,18-dehydroexcelsin and glaucarubol from Ailanthus excelsa. Phytochemistry. 1980;19:2484–5.
19. Khan S, Zuberi S, Shamsuddin K. Isolation and structure of excelsin. Indian J Chem B. 1980;19:183–7.
20. Kumar D, et al. Ailanthus excelsa Roxb. is really a plant of heaven. Int J Pharmacol. 2010;6(5):535–50.
21. Paech K, Tracey MV. Modern Methods of Plant Analysis. Vol. III. Berlin: Springer-Verlag; 1955. p. 626.
22. Harborne JB. Phytochemical Methods. London: Chapman and Hall; 1999. p. 60–6.
23. Trease GE, Evans WC. Textbook of Pharmacognosy. 12th ed. London: Balliere Tindall; 1983. p. 257.
24. Loomis WE, Shull CA. Methods in plant physiology. In: Sinnot MW, editor. McGraw-Hill Botanical Science Series. New York: McGraw-Hill; 1937. p. 290.
25. McCready MR, et al. Determination of starch and amylose in vegetables. Anal Chem. 1950;22:1156–8.
26. Dubois M, et al. A colorimetric method for the determination of sugar. Nature. 1951;168:167.
27. Osborn DJ. Effect of kinetin on protein and nucleic acid metabolism in Xanthium leaves. Plant Physiol. 1962;37:595–602.
28. Lowry OH, et al. Protein measurement with the Folin-phenol reagent. J Biol Chem. 1951;193:265–75.
29. Jayaraman J. Laboratory Manual in Biochemistry. New Delhi: Wiley Eastern; 1958. p. 96–7.
30. Bray HG, Thorpe WV. Analysis of phenolic compounds. Methods Biochem Anal. 1954;1:27–52.
31. Roe JH, Kuenther CA. Determination of ascorbic acid in blood and urine using 2,4-nitrophenylhydrazine. J Biol Chem. 1943;147:399–407.
32. Sunderland N. Pigmented plant tissues in culture. Ann Bot. 1966;30:253–68.
33. Holden M. Chlorophylls. In: Goodwin TW, editor. Chemistry and Biochemistry of Plant Pigments. London: Academic Press; 1976. p. 462–88.
34. Arnon DI. Copper enzymes in isolated chloroplasts. Plant Physiol. 1949;24:1–5.
35. Singh R, Muftah AMS, Belkheir A. Antibacterial and antioxidant activities of Mentha piperita L. Arab J Chem. 2015;8:322–8.
36. Murugan N, Mohan VR. Antibacterial activity of Mucuna pruriens (L.) DC. var. pruriens. J Sci Res Rep. 2011;1(2):69–72.
37. Sofowora A. Medicinal Plants and Traditional Medicine in Africa. Ibadan: Spectrum Books; 1993.
38. Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. London: Chapman and Hall; 1998.
39. Ogunleye DS, Ibitoye SF. Studies on antimicrobial activity and chemical constituents of Ximenia americana. Trop J Pharm Res. 2003;2:239–41.
40. Sodipo OA, et al. Saponin is the active antifungal principle in Garcinia kola seed. Biosci Res Commun. 1991;3:171–2.
41. Stephan UA, et al. Phytochemical analysis and antibacterial activity of Khaya grandifolia stem bark. J Biol Sci. 2009;9(1):63–7.
42. Okeke AO. Herbal treatment to reduce dental caries with Newbouldia laevis extract. Am J Undergrad Res. 2003;2:1–4.
43. Kokate CK, Purohit AP, Gokhale SB. Practical Pharmacognosy. Pune: Nirali Prakashan; 2003.

Please cite this article in press as Aruna Kumari et al. Preliminary Phytochemical Analysis And Quantitative Estimation of Primary Metabolites of Leaf And Stem of Ailanthus Excelsa Roxb. Indo American Journal of Pharmaceutical Research.2025:15(06).